nudF [2019-11-21 16:36:28]

involved in isopentenol (isoprenoid) biosynthesis

locus: BSU_23610

pI: 4.59

mw: 20.84 kDa

protein length: 185 aa

gene length: 558 bp

function: isoprenoid biosynthesis

product: unknown

essential: no

ec: 3.6.1.13

synonyms: yqkG

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids]

Gene

Coordinates: 2,458,509 → 2,459,066

The protein

Catalyzed reaction/ biological activity

• ADP-D-ribose + H₂O --> AMP + D-ribose 5-phosphate + 2 H⁺ (according to UniProt)

Protein family

• [SW|Nudix hydrolase] (according to UniProt)

[SW|Domains]

• [SW|Nudix hydrolase domain] (aa 40-171) (according to UniProt)

Structure

• [PDB|1V8I] (from ''Thermus thermophilus'', 38% identity) [Pubmed|15210687]

Expression and Regulation

Operons

• genes: [gene|CF38BE04F04919BB304789E9B3FE489385E468E6|nudF]-[gene|9D05B104000EED08FB62D6F362FC5E9C2A5354D9|yqxK]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-C410 (yqkG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2408 NBRP B. subtilis, Japan]
• BKE23610 (Δ[gene|CF38BE04F04919BB304789E9B3FE489385E468E6|nudF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTCATCTCCCGTTC, downstream forward: _UP4_AAAGAAGCGCTCCAAGCACA
• BKK23610 (Δ[gene|CF38BE04F04919BB304789E9B3FE489385E468E6|nudF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTCATCTCCCGTTC, downstream forward: _UP4_AAAGAAGCGCTCCAAGCACA

References

• 17693564,10542272,15210687